Hotspot

ndhC-trnV-UAC

LSC · 52979-54168 · 1190 bp

Back to Circaea

Pi 0.00927

MarkerSeek score 56.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1190
variable_sites: 31
pi: 0.00927
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.9	58.7	1202-1234 bp	1.0	44.7
ndhC-trnV-UAC_p2	TCATATTCGTAAAGCAGAAACA	TTCGAGTCCGTATAGCCCTA	31.8	50.0	53.9	57.1	1201-1233 bp	1.0	44.7
ndhC-trnV-UAC_p3	TCATATTCGTAAAGCAGAAACAT	GTTCGAGTCCGTATAGCCCT	30.4	55.0	54.5	58.7	1202-1234 bp	1.0	44.7
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGAAACA	AGGTCTACGGTTCGAGTCC	31.8	57.9	53.9	58.1	1211-1243 bp	1.0	44.7
ndhC-trnV-UAC_p5	CATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	33.3	55.0	52.3	58.7	1201-1233 bp	1.0	44.7