Hotspot

trnF-GAA-ndhJ

LSC · 50557-51305 · 749 bp

Back to Circaea

Pi 0.0141

MarkerSeek score 68.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 35
pi: 0.0141
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	854-875 bp	1.0	82.4
trnF-GAA-ndhJ_p2	TCAAATCTGGTTCCTGGCAC	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	839-860 bp	1.0	82.2
trnF-GAA-ndhJ_p3	TAAGGAAGGGTCGGGATAGC	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.3	58.1	902-923 bp	1.0	80.4
trnF-GAA-ndhJ_p4	CCTAAGGAAGGGTCGGGATA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.6	58.1	904-925 bp	1.0	79.5
trnF-GAA-ndhJ_p5	TCGGGATAGCTCAGTTGGTA	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.6	58.1	892-913 bp	1.0	79.1