Hotspot

psbM-trnD-GUC

LSC · 30722-31839 · 1118 bp

Back to Circaea

Pi 0.0092

MarkerSeek score 54.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1118
variable_sites: 28
pi: 0.0092
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CCCGAGACGAAAAGCCTTTA	GAGCATAGTATGAGAGCGCC	50.0	55.0	57.9	58.0	1993-2034 bp	1.0	81.8
psbM-trnD-GUC_p2	ATTCGAGTCCGCCAATTTCA	GAGCATAGTATGAGAGCGCC	45.0	55.0	57.9	58.0	2020-2061 bp	1.0	81.6
psbM-trnD-GUC_p3	TCGAGTCCGCCAATTTCATT	GAGCATAGTATGAGAGCGCC	45.0	55.0	57.9	58.0	2018-2059 bp	1.0	81.6
psbM-trnD-GUC_p4	TGATTCGAGTCCGCCAATTT	GAGCATAGTATGAGAGCGCC	45.0	55.0	57.9	58.0	2022-2063 bp	1.0	81.6
psbM-trnD-GUC_p5	TTCGAGTCCGCCAATTTCAT	GAGCATAGTATGAGAGCGCC	45.0	55.0	57.9	58.0	2019-2060 bp	1.0	81.6