Hotspot

trnC-GCA-petN

LSC · 29051-29948 · 898 bp

Back to Circaea

Pi 0.00672

MarkerSeek score 63.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 898
variable_sites: 18
pi: 0.00672
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAATGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.1	951-962 bp	1.0	81.2
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	TTAATGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.1	969-980 bp	1.0	79.0
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TACCTCTAGAGCCCACTCCT	50.0	55.0	58.1	58.4	1012-1023 bp	1.0	78.6
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	GCCCAAGCAAGACTGACTAT	50.0	50.0	58.1	57.6	943-954 bp	1.0	78.6
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TTACCTCTAGAGCCCACTCC	50.0	55.0	58.1	57.5	1013-1024 bp	1.0	78.1