Hotspot

trnS-GCU-trnG-GCC

LSC · 8564-9369 · 806 bp

Back to Circaea

Pi 0.0122
MarkerSeek score 64.30
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
806
variable_sites
29
pi
0.0122
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 843-882 bp 1.0 58.3
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 842-881 bp 1.0 58.3
trnS-GCU-trnG-GCC_p3 ACGGAAAGAGAGGGATTCGA ACGAATCACACTTTTACCACT 50.0 38.1 58.2 55.5 897-936 bp 1.0 57.0
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA AACGAATCACACTTTTACCACT 50.0 36.4 58.0 56.2 844-883 bp 1.0 56.4
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC AACGAATCACACTTTTACCACT 50.0 36.4 58.0 56.2 843-882 bp 1.0 56.4