Hotspot

ycf1

Genome · 128390-134029 · 5640 bp

Back to Cinnamomum

Pi 0.00607

MarkerSeek score 45.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5640
variable_sites: 149
pi: 0.00607
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGCCTCTTCCTCCTCAGAA	TGGCTTTGGTACGTTACTCG	50.0	50.0	58.0	57.9	255-264 bp	1.0	84.2
ycf1_p2	TTTGCCTCTTCCTCCTCAGA	TGGCTTTGGTACGTTACTCG	50.0	50.0	58.0	57.9	256-265 bp	1.0	84.2
ycf1_p3	TTGCCTCTTCCTCCTCAGAA	TACGCGCTCAAAGACGTAAA	50.0	45.0	58.0	58.0	195-204 bp	0.967742	83.4
ycf1_p4	TTTGCCTCTTCCTCCTCAGA	TACGCGCTCAAAGACGTAAA	50.0	45.0	58.0	58.0	196-205 bp	0.967742	83.4
ycf1_p5	TTGCCTCTTCCTCCTCAGAA	GATCCATACGCGCTCAAAGA	50.0	50.0	58.0	58.1	201-210 bp	0.967742	82.5