Hotspot

rpl2-trnI-CAU

Genome · 89332-89747 · 416 bp

Back to Cinnamomum

Pi 0.0101

MarkerSeek score 48.20

Primer available yes

Label rpl2-trnI-CAU

Feature Metadata

label_name: rpl2-trnI-CAU
feature_type: igs
region: Genome
length_bp: 416
variable_sites: 15
pi: 0.0101
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnI-CAU_p1	TGGATCCTTTGCGTATGCTC	ACTCGCGATGTACAATGACC	50.0	50.0	58.0	58.1	559-570 bp	1.0	83.0
rpl2-trnI-CAU_p2	TGGATCCTTTGCGTATGCTC	GGTCACTCGCGATGTACAAT	50.0	50.0	58.0	58.1	563-574 bp	1.0	83.0
rpl2-trnI-CAU_p3	TGGATCCTTTGCGTATGCTC	AATGACCCCAGTGAAGCATC	50.0	50.0	58.0	57.9	546-557 bp	1.0	82.6
rpl2-trnI-CAU_p4	TGGATCCTTTGCGTATGCTC	CAATGACCCCAGTGAAGCAT	50.0	50.0	58.0	58.2	547-558 bp	1.0	82.4
rpl2-trnI-CAU_p5	TGCTCGGGGTAGAAGTTTTG	ACTCGCGATGTACAATGACC	50.0	50.0	57.8	58.1	544-555 bp	1.0	82.0