Hotspot

rps19-rpl2

Genome · 87755-87833 · 79 bp

Back to Cinnamomum

Pi 0.0224

MarkerSeek score 67.20

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: Genome
length_bp: 79
variable_sites: 7
pi: 0.0224
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCCCGGGACCAAGTTACTAT	GTTATCCTGCGCTTGGAAGA	55.0	50.0	59.2	58.0	261-271 bp	1.0	76.2
rps19-rpl2_p2	GCCCGGGACCAAGTTACTAT	CCTGCGCTTGGAAGAAAAAG	55.0	50.0	59.2	57.9	256-266 bp	1.0	76.1
rps19-rpl2_p3	GCCCGGGACCAAGTTACTAT	ATCCTGCGCTTGGAAGAAAA	55.0	45.0	59.2	58.1	258-268 bp	1.0	75.8
rps19-rpl2_p4	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	275-285 bp	1.0	74.9
rps19-rpl2_p5	GCCCGGGACCAAGTTACTAT	GGCCCCGATTGGTAGAAAAA	55.0	50.0	59.2	58.2	299-309 bp	1.0	74.7