Hotspot

petA-psbJ

Genome · 66343-67502 · 1160 bp

Back to Cinnamomum

Pi 0.0157

MarkerSeek score 61.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 1160
variable_sites: 57
pi: 0.0157
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	GGCTCATATTCCGGATTGGG	45.0	55.0	58.2	58.2	1271-1388 bp	1.0	84.8
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	CGGCTCATATTCCGGATTGG	45.0	55.0	58.2	58.6	1272-1389 bp	1.0	81.7
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	GCTCATATTCCGGATTGGGA	45.0	50.0	58.2	57.1	1270-1387 bp	1.0	79.3
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	58.2	57.1	1264-1381 bp	1.0	79.1
petA-psbJ_p5	CGTGTCCAAGGTTTGTTGTT	GGCTCATATTCCGGATTGGG	45.0	55.0	57.1	58.2	1270-1387 bp	1.0	78.7