Hotspot

ndhC-trnV-UAC

Genome · 52601-54451 · 1851 bp

Back to Cinnamomum

Pi 0.00191

MarkerSeek score 48.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: Genome
length_bp: 1851
variable_sites: 20
pi: 0.00191
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTTCACTAATCGGGGCCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1987-2008 bp	1.0	78.6
ndhC-trnV-UAC_p2	ACTAATCGGGGCCAAAACTC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1983-2004 bp	1.0	78.6
ndhC-trnV-UAC_p3	CACTAATCGGGGCCAAAACT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1984-2005 bp	1.0	78.3
ndhC-trnV-UAC_p4	TCACTAATCGGGGCCAAAAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1985-2006 bp	1.0	78.3
ndhC-trnV-UAC_p5	CTTCACTAATCGGGGCCAAA	ACGCATGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	2053-2074 bp	1.0	77.7