Hotspot

psbC-trnS-UGA

Genome · 37592-37805 · 214 bp

Back to Cinnamomum

Pi 0.0218

MarkerSeek score 47.40

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: Genome
length_bp: 214
variable_sites: 12
pi: 0.0218
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	336-337 bp	1.0	81.4
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GGCTGAGTGGTTGATAGCTC	55.0	55.0	58.2	58.1	329-330 bp	1.0	81.2
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	AGCTCTGGTCTTGAAAACCG	55.0	50.0	58.2	58.1	314-315 bp	1.0	80.8
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.2	58.2	267-268 bp	1.0	80.6
psbC-trnS-UGA_p5	AGCTGCAGCAGGATTTGAAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.4	58.0	379-380 bp	1.0	79.4