Hotspot

trnS-GCU-trnG-UCC

Genome · 9498-10315 · 818 bp

Back to Cinnamomum

Pi 0.00347

MarkerSeek score 53.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: Genome
length_bp: 818
variable_sites: 13
pi: 0.00347
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	AGGAATCAAACGAAAGATCCCT	45.0	40.9	58.0	57.4	814-943 bp	1.0	64.2
trnS-GCU-trnG-UCC_p2	GCTTTAGTCCACTCAGCCAT	AGGAATCAAACGAAAGATCCCT	50.0	40.9	57.6	57.4	800-929 bp	1.0	61.2
trnS-GCU-trnG-UCC_p3	AGAAGAAACGGAGAGAGGGG	AGGAATCAAACGAAAGATCCCT	55.0	40.9	58.4	57.4	870-999 bp	1.0	61.0
trnS-GCU-trnG-UCC_p4	AGTCCACTCAGCCATCTCTC	AGGAATCAAACGAAAGATCCCT	55.0	40.9	58.5	57.4	795-924 bp	1.0	60.4
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	GGAATCAAACGAAAGATCCCT	45.0	42.9	58.0	55.9	813-942 bp	1.0	60.3