Hotspot

trnK-UUU-rps16

Genome · 4357-5181 · 825 bp

Back to Cinnamomum

Pi 0.0035

MarkerSeek score 49.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: Genome
length_bp: 825
variable_sites: 16
pi: 0.0035
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	945-957 bp	1.0	81.7
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	944-956 bp	1.0	81.7
trnK-UUU-rps16_p3	AAAAGACCATAGCCGCACTT	AAAAAGGCGCTCAACCTACA	45.0	45.0	57.8	58.0	966-978 bp	1.0	81.4
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	954-966 bp	1.0	80.8
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.2	58.2	948-960 bp	1.0	80.1