Hotspot

rpl22

IRa · 155639-156106 · 468 bp

Back to Cinchona

Pi 0.0256

MarkerSeek score 45.90

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: IRa
length_bp: 468
variable_sites: 16
pi: 0.0256
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.06
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TGGGTAGAGCCGGATCTAAG	ATCCATTCGACACGTGCAAT	55.0	45.0	58.0	58.3	1589-1612 bp	0.5	58.3
rpl22_p2	AACAGTCGGACAAGTAGGGA	ATCCATTCGACACGTGCAAT	50.0	45.0	58.0	58.3	1633-1656 bp	0.5	58.3
rpl22_p3	ACAGTCGGACAAGTAGGGAA	ATCCATTCGACACGTGCAAT	50.0	45.0	58.0	58.3	1632-1655 bp	0.5	58.3
rpl22_p4	AGTAGGGAATGTTGGGGTGA	ATCCATTCGACACGTGCAAT	50.0	45.0	57.9	58.3	1621-1644 bp	0.5	57.9
rpl22_p5	TGGGTAGAGCCGGATCTAAG	CCATTCGACACGTGCAATTT	55.0	45.0	58.0	57.7	1587-1610 bp	0.5	57.8