Hotspot

rpl16

LSC · 82827-84227 · 1401 bp

Back to Cinchona

Pi 0.0069

MarkerSeek score 50.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1401
variable_sites: 18
pi: 0.0069
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CATTTCTCTTGGTTCTACTTTAA	TAACCGTTTGATAAAATTGCT	30.4	28.6	52.4	52.0	1448-1453 bp	1.0	41.3
rpl16_p2	CATTTCTCTTGGTTCTACTTTAA	TTAACCGTTTGATAAAATTGCT	30.4	27.3	52.4	52.9	1449-1454 bp	1.0	41.3
rpl16_p3	TCTCTTGGTTCTACTTTAATTTATCCT	TAACCGTTTGATAAAATTGCT	29.6	28.6	56.3	52.0	1444-1449 bp	1.0	41.3
rpl16_p4	TCTCTTGGTTCTACTTTAATTTATCCT	TTAACCGTTTGATAAAATTGCT	29.6	27.3	56.3	52.9	1445-1450 bp	1.0	41.3
rpl16_p5	CATTTCTCTTGGTTCTACTTTAA	GATTAACCGTTTGATAAAATTGCT	30.4	29.2	52.4	54.8	1451-1456 bp	1.0	41.3