Hotspot

pafI

LSC · 43722-45698 · 1977 bp

Back to Cinchona

Pi 0.00354

MarkerSeek score 60.10

Primer available yes

Label pafI

Feature Metadata

label_name: pafI
feature_type: gene
region: LSC
length_bp: 1977
variable_sites: 12
pi: 0.00354
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI_p1	TGATGGGGCCAACAAACAAT	AGTGGGATCATTTTCTCGCG	45.0	50.0	58.3	58.3	2154-2162 bp	1.0	75.5
pafI_p2	TGATGGGGCCAACAAACAAT	GTGGGATCATTTTCTCGCGA	45.0	50.0	58.3	58.3	2153-2161 bp	1.0	75.5
pafI_p3	TGATGGGGCCAACAAACAAT	TGGGATCATTTTCTCGCGAA	45.0	45.0	58.3	57.6	2152-2160 bp	1.0	75.1
pafI_p4	TGGGGCCAACAAACAATTCA	AGTGGGATCATTTTCTCGCG	45.0	50.0	58.5	58.3	2151-2159 bp	1.0	73.7
pafI_p5	TGGGGCCAACAAACAATTCA	GTGGGATCATTTTCTCGCGA	45.0	50.0	58.5	58.3	2150-2158 bp	1.0	73.7