Hotspot

ndhF

SSC · 111509-113734 · 2226 bp

Back to Cinchona

Pi 0.00539

MarkerSeek score 47.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 20
pi: 0.00539
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	ACCTCCCTGTCTAGTTTCGT	50.0	50.0	58.0	57.7	2944-2955 bp	1.0	78.1
ndhF_p2	AAGAGGGATCCACCGAAGAA	ACCTCCCTGTCTAGTTTCGT	50.0	50.0	58.0	57.7	2936-2947 bp	1.0	78.1
ndhF_p3	AGGGATCCACCGAAGAAGAT	ACCTCCCTGTCTAGTTTCGT	50.0	50.0	57.8	57.7	2933-2944 bp	1.0	77.0
ndhF_p4	CAGGAACAAGAGGGATCCAC	ACCTCCCTGTCTAGTTTCGT	55.0	50.0	57.6	57.7	2943-2954 bp	1.0	75.2
ndhF_p5	ACTTCCGAAATGAAGGGGAC	ACCTCCCTGTCTAGTTTCGT	50.0	50.0	57.5	57.7	2967-2978 bp	1.0	74.6