Hotspot

atpB

LSC · 54446-55942 · 1497 bp

Back to Cinchona

Pi 0.00189

MarkerSeek score 58.20

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 5
pi: 0.00189
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CATGGTTGGGTAATACGCCA	AATTAACTGATCGACGCGCT	50.0	45.0	58.0	58.1	1693 bp	1.0	79.3
atpB_p2	CATGGTTGGGTAATACGCCA	GAATTAACTGATCGACGCGC	50.0	50.0	58.0	57.7	1694 bp	1.0	77.7
atpB_p3	CATGGTTGGGTAATACGCCA	TAACTGATCGACGCGCTATC	50.0	50.0	58.0	57.7	1690 bp	1.0	77.4
atpB_p4	GGGGCATGGTTGGGTAATAC	AATTAACTGATCGACGCGCT	55.0	45.0	58.3	58.1	1697 bp	1.0	77.2
atpB_p5	CATGGTTGGGTAATACGCCA	CGACGCGCTATCGGATATTT	50.0	50.0	58.0	58.4	1682 bp	1.0	76.8