Hotspot

ccsA-ndhD

SSC · 116710-116979 · 270 bp

Back to Cinchona

Pi 0.0377

MarkerSeek score 72.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 270
variable_sites: 17
pi: 0.0377
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACTCAGCAATTGTAGCGTCT	ATCGGTTGACAAGGTCGAAG	45.0	50.0	57.2	57.9	435 bp	1.0	76.5
ccsA-ndhD_p2	TTAGGAATAGGGCTGCACAG	ATCGGTTGACAAGGTCGAAG	50.0	50.0	57.0	57.9	370 bp	1.0	75.0
ccsA-ndhD_p3	AACTCAGCAATTGTAGCGTCT	ATCGGTTGACAAGGTCGAAG	42.9	50.0	58.0	57.9	436 bp	1.0	74.1
ccsA-ndhD_p4	ACTCAGCAATTGTAGCGTCT	CGGTTGACAAGGTCGAAGTT	45.0	50.0	57.2	58.4	433 bp	1.0	73.8
ccsA-ndhD_p5	ACTCAGCAATTGTAGCGTCT	ACTATCGGTTGACAAGGTCG	45.0	50.0	57.2	57.4	438 bp	1.0	71.9