Hotspot

rpl32-trnL-UAG

SSC · 114705-115572 · 868 bp

Back to Cinchona

Pi 0.0144
MarkerSeek score 57.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
868
variable_sites
22
pi
0.0144
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGAAGGGATATTGGACAGCG AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 985-995 bp 1.0 81.7
rpl32-trnL-UAG_p2 GGAAGGGATATTGGACAGCG CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 986-996 bp 1.0 81.7
rpl32-trnL-UAG_p3 GGAAGGGATATTGGACAGCG TCGAACCGAGATGCTTTAGC 55.0 50.0 58.1 58.1 1021-1031 bp 1.0 81.0
rpl32-trnL-UAG_p4 GGAAGGGATATTGGACAGCG TATCATGCCCTTTTCGGACG 55.0 50.0 58.1 58.1 1265-1275 bp 1.0 80.9
rpl32-trnL-UAG_p5 GGAAGGGATATTGGACAGCG AGCCTTGGGTAAAAACAGCA 55.0 45.0 58.1 57.9 1461-1471 bp 1.0 80.4