Hotspot

ndhF-rpl32

SSC · 113735-114542 · 808 bp

Back to Cinchona

Pi 0.00784

MarkerSeek score 59.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 808
variable_sites: 12
pi: 0.00784
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCCGTATTGGTATGGCTGTT	CGCTGTCCAATATCCCTTCC	50.0	55.0	57.7	58.1	906-917 bp	1.0	78.3
ndhF-rpl32_p2	CACCCGTATTGGTATGGCTG	CGCTGTCCAATATCCCTTCC	55.0	55.0	58.4	58.1	908-919 bp	1.0	77.7
ndhF-rpl32_p3	CGTATTGGTATGGCTGTTGC	CGCTGTCCAATATCCCTTCC	50.0	55.0	57.2	58.1	904-915 bp	1.0	75.0
ndhF-rpl32_p4	ACCCGTATTGGTATGGCTGT	CGCTGTCCAATATCCCTTCC	50.0	55.0	58.8	58.1	907-918 bp	1.0	74.7
ndhF-rpl32_p5	CCCGTATTGGTATGGCTGTT	ACGCTGTCCAATATCCCTTC	50.0	50.0	57.7	57.4	907-918 bp	1.0	74.2