Hotspot

petB-petD

LSC · 78024-78868 · 845 bp

Back to Cinchona

Pi 0.00947

MarkerSeek score 61.90

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 845
variable_sites: 13
pi: 0.00947
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	CCGCTTCTTACTGCCGTATT	TGACCCATACCTTTAGCCAA	50.0	45.0	58.1	56.1	1033 bp	1.0	65.4
petB-petD_p2	AAACAGGGTATTTCTGGCCC	TGACCCATACCTTTAGCCAA	50.0	45.0	57.8	56.1	985 bp	1.0	64.1
petB-petD_p3	CCGCTTCTTACTGCCGTATT	ATGACCCATACCTTTAGCCA	50.0	45.0	58.1	55.9	1034 bp	1.0	63.5
petB-petD_p4	CCGCTTCTTACTGCCGTATT	TCTTATAGATCCATCCCCCGA	50.0	47.6	58.1	56.9	954 bp	1.0	63.5
petB-petD_p5	TATTGCCGCTTCTTACTGCC	TGACCCATACCTTTAGCCAA	50.0	45.0	58.3	56.1	1038 bp	1.0	63.2