Hotspot

ndhC-trnV-UAC

LSC · 51674-52906 · 1233 bp

Back to Cinchona

Pi 0.013
MarkerSeek score 63.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1233
variable_sites
27
pi
0.013
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGCCAAAATAGGAATAAGACTTGA AGAAGGTCTACGGTTCGAGT 37.5 50.0 57.0 57.8 1248-1338 bp 1.0 45.7
ndhC-trnV-UAC_p2 AGGCCAAAATAGGAATAAGACTTG AGAAGGTCTACGGTTCGAGT 37.5 50.0 57.0 57.8 1249-1339 bp 1.0 45.7
ndhC-trnV-UAC_p3 AGGCCAAAATAGGAATAAGACT AGAAGGTCTACGGTTCGAGT 36.4 50.0 54.8 57.8 1249-1339 bp 1.0 45.7
ndhC-trnV-UAC_p4 AGGCCAAAATAGGAATAAGACTTGA AGAAGGTCTACGGTTCGAGT 36.0 50.0 58.3 57.8 1249-1339 bp 1.0 45.7
ndhC-trnV-UAC_p5 GGCCAAAATAGGAATAAGACTTGA GAAGGTCTACGGTTCGAGTC 37.5 55.0 57.0 57.5 1247-1337 bp 1.0 45.7