Hotspot

rpoB-trnC-GCA

LSC · 27268-28323 · 1056 bp

Back to Cinchona

Pi 0.0101

MarkerSeek score 59.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1056
variable_sites: 20
pi: 0.0101
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.0	58.1	1139 bp	1.0	72.6
rpoB-trnC-GCA_p2	AGCCATTCCCTCATTTCCATC	CCGGATTTGAACTGGGGAAA	47.6	50.0	58.0	58.1	1148 bp	1.0	72.4
rpoB-trnC-GCA_p3	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1144 bp	1.0	72.1
rpoB-trnC-GCA_p4	CCAGGTATTGTAGCCATTCCC	CCGGATTTGAACTGGGGAAA	52.4	50.0	58.1	58.1	1159 bp	1.0	71.6
rpoB-trnC-GCA_p5	TCATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.2	58.1	1138 bp	1.0	71.5