Hotspot

trnG-GCC-trnR-UCU

LSC · 10145-10317 · 173 bp

Back to Cinchona

Pi 0.00963

MarkerSeek score 59.50

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 173
variable_sites: 3
pi: 0.00963
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	251-252 bp	1.0	64.6
trnG-GCC-trnR-UCU_p2	ACCCTTAGCCTTCCAAGCTA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	257-258 bp	1.0	64.6
trnG-GCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	257-258 bp	1.0	63.9
trnG-GCC-trnR-UCU_p4	ACCCTTAGCCTTCCAAGCTA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	263-264 bp	1.0	63.9
trnG-GCC-trnR-UCU_p5	TCGACGTCGACTATAACCCT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.7	56.9	272-273 bp	1.0	62.2