Hotspot

rps16-trnQ-UUG

LSC · 6116-7564 · 1449 bp

Back to Cinchona

Pi 0.0105

MarkerSeek score 56.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1449
variable_sites: 26
pi: 0.0105
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GATTCCCGCGTGATACACTT	CTTTTGACCCCACCCAATCA	50.0	50.0	58.1	58.0	1943-2115 bp	1.0	84.6
rps16-trnQ-UUG_p2	ACTTCGGTGCTTCTGAATCC	CTTTTGACCCCACCCAATCA	50.0	50.0	57.9	58.0	2029-2201 bp	1.0	84.2
rps16-trnQ-UUG_p3	GATTCCCGCGTGATACACTT	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.1	58.1	2212-2384 bp	1.0	84.1
rps16-trnQ-UUG_p4	GATTCCCGCGTGATACACTT	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.1	57.9	2217-2389 bp	1.0	84.1
rps16-trnQ-UUG_p5	TGAGTGGTCTTTACCCCCTT	CTTTTGACCCCACCCAATCA	50.0	50.0	57.9	58.0	2169-2341 bp	1.0	84.0