Hotspot

rpoC1

LSC · 33770-36575 · 2806 bp

Back to Cichorium

Pi 0.00249

MarkerSeek score 57.50

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2806
variable_sites: 7
pi: 0.00249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	ACGATCTTTAGCTCTGGAACTG	ACAATGGGCTTGAGTCAGTG	45.5	50.0	57.9	58.1	2989-2990 bp	1.0	62.5
rpoC1_p2	TCTGAGAAGACTTTCCAGCT	ACAATGGGCTTGAGTCAGTG	45.0	50.0	55.8	58.1	2952-2953 bp	1.0	61.8
rpoC1_p3	ACGATCTTTAGCTCTGGAACTG	CAATGGGCTTGAGTCAGTGA	45.5	50.0	57.9	57.8	2988-2989 bp	1.0	61.7
rpoC1_p4	ACGATCTTTAGCTCTGGAACTG	TGGGCTTGAGTCAGTGATTG	45.5	50.0	57.9	57.8	2985-2986 bp	1.0	61.7
rpoC1_p5	GTTCGTTCGAGAACTACGATCT	TGGGCTTGAGTCAGTGATTG	45.5	50.0	58.0	57.8	3000 bp	0.5	42.5