Hotspot

accD

LSC · 74703-76109 · 1407 bp

Back to Cichorium

Pi 0.0156

MarkerSeek score 56.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1407
variable_sites: 22
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCATGCAAATATGGAGCAAGA	ACTTGCTTGCTCAAAATAATTGA	38.1	30.4	55.6	55.6	1475-1487 bp	1.0	41.2
accD_p2	TCATGCAAATATGGAGCAAGA	TGCTTGCTCAAAATAATTGAACT	38.1	30.4	55.6	55.6	1472-1484 bp	1.0	41.2
accD_p3	TCATGCAAATATGGAGCAAGA	ACTTGCTTGCTCAAAATAATTGAAC	38.1	32.0	55.6	57.6	1475-1487 bp	1.0	41.2
accD_p4	TCATGCAAATATGGAGCAAGAA	ACTTGCTTGCTCAAAATAATTGA	36.4	30.4	56.4	55.6	1475-1487 bp	1.0	41.2
accD_p5	TCATGCAAATATGGAGCAAGAA	TGCTTGCTCAAAATAATTGAACT	36.4	30.4	56.4	55.6	1472-1484 bp	1.0	41.2