Hotspot

atpB-rbcL

LSC · 71976-72733 · 758 bp

Back to Cichorium

Pi 0.00396

MarkerSeek score 61.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 758
variable_sites: 3
pi: 0.00396
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	918-919 bp	1.0	72.0
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	917-918 bp	1.0	71.7
atpB-rbcL_p3	CGCCCCAGTTTCTTTTTGTC	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	871-872 bp	1.0	71.2
atpB-rbcL_p4	CGCCCCAGTTTCTTTTTGTC	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.9	58.1	870-871 bp	1.0	70.9
atpB-rbcL_p5	TTCTTTTTGTCAAGCGTGGC	CACCAGCTTTGAATCCAACAC	45.0	47.6	57.8	58.0	862-863 bp	1.0	70.6