Hotspot

trnT-TGT-trnL-UAA

LSC · 63979-64539 · 561 bp

Back to Cichorium

Pi 0.00899

MarkerSeek score 64.00

Primer available yes

Label trnT-TGT-trnL-UAA

Feature Metadata

label_name: trnT-TGT-trnL-UAA
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 5
pi: 0.00899
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-TGT-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	622-629 bp	1.0	78.2
trnT-TGT-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	654-661 bp	1.0	77.3
trnT-TGT-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	616-623 bp	1.0	76.9
trnT-TGT-trnL-UAA_p4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	648-655 bp	1.0	76.0
trnT-TGT-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	55.0	55.0	57.9	58.6	621-628 bp	1.0	75.3