Hotspot

trnC-GCA-petN

LSC · 27081-27914 · 834 bp

Back to Cichorium

Pi 0.0122

MarkerSeek score 67.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 10
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	891-893 bp	1.0	80.5
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	909-911 bp	1.0	78.2
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	890-892 bp	1.0	74.5
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	899-901 bp	1.0	74.4
trnC-GCA-petN_p5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.1	58.2	948-950 bp	1.0	73.7