Hotspot

ycf1

SSC · 122983-127989 · 5007 bp

Back to Cicerbita

Pi 0.023

MarkerSeek score 54.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5007
variable_sites: 197
pi: 0.023
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGAGGAGGACCAAAAGGAT	GAAATCCTCATTCCCCGCTT	50.0	50.0	58.0	57.9	584 bp	1.0	83.7
ycf1_p2	CGAGGAGGACCAAAAGGATG	GAAATCCTCATTCCCCGCTT	55.0	50.0	58.0	57.9	583 bp	0.5	59.6
ycf1_p3	CGAGGAGGACCAAAAGGATG	ACCAGATTTTCGTCGATCCG	55.0	50.0	58.0	58.1	663 bp	0.5	59.4
ycf1_p4	CGAGGAGGACCAAAAGGATG	CCAGATTTTCGTCGATCCGT	55.0	50.0	58.0	58.1	662 bp	0.5	59.4
ycf1_p5	TCGAGGAGGACCAAAAGGAT	CCAGATTTTCGTCGATCCGT	50.0	50.0	58.0	58.1	663 bp	0.5	59.2