Hotspot

rps3

LSC · 82569-83225 · 657 bp

Back to Cicerbita

Pi 0.00431

MarkerSeek score 38.20

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 5
pi: 0.00431
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	ATTGGTTTCTGGTCGGTTCC	AAGCGCGAAGGGTAATTGAT	50.0	45.0	58.1	58.0	1786-1797 bp	1.0	80.6
rps3_p2	ATTGGTTTCTGGTCGGTTCC	TCCCGGGCATCTACCATTAT	50.0	50.0	58.1	58.0	2091-2102 bp	1.0	80.5
rps3_p3	ATTGGTTTCTGGTCGGTTCC	GAGGGCGTAGTTTTGCGATA	50.0	50.0	58.1	58.1	1564-1575 bp	1.0	80.5
rps3_p4	TTGTTTATACCGTGGGGCTG	AAGCGCGAAGGGTAATTGAT	50.0	45.0	57.9	58.0	1271 bp	1.0	79.8
rps3_p5	TTGTTTATACCGTGGGGCTG	TCCCGGGCATCTACCATTAT	50.0	50.0	57.9	58.0	1576-1578 bp	1.0	79.8