Hotspot

rpl32-trnL

SSC · 112522-113413 · 892 bp

Back to Cicerbita

Pi 0.0239

MarkerSeek score 70.30

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 892
variable_sites: 36
pi: 0.0239
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	AAGGAAAGGATATTGGGCGG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	1022-1062 bp	1.0	82.0
rpl32-trnL_p2	AAGGAAAGGATATTGGGCGG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	1023-1063 bp	1.0	81.9
rpl32-trnL_p3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.6	58.0	1005-1045 bp	1.0	81.8
rpl32-trnL_p4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.6	58.0	1006-1046 bp	1.0	81.8
rpl32-trnL_p5	AAGGAAAGGATATTGGGCGG	TTAGAAGATGTCATGCCGCC	50.0	50.0	57.6	58.0	1086-1126 bp	1.0	81.4