Hotspot

ndhF-rpl32

SSC · 111300-112356 · 1057 bp

Back to Cicerbita

Pi 0.017

MarkerSeek score 70.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1057
variable_sites: 30
pi: 0.017
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CCGCCCAATATCCTTTCCTT	50.0	50.0	58.0	57.6	1233-1243 bp	1.0	79.5
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CCGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	1231-1241 bp	1.0	79.5
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CCGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	1232-1242 bp	1.0	79.4
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	50.0	45.0	58.0	57.6	1250-1260 bp	1.0	79.3
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	45.0	45.0	58.0	57.6	1248-1258 bp	1.0	79.2