Hotspot

rps12-ycf15

IRb · 97919-98936 · 1018 bp

Back to Cicerbita

Pi 0.00378

MarkerSeek score 64.60

Primer available yes

Label rps12-ycf15

Feature Metadata

label_name: rps12-ycf15
feature_type: igs
region: IRb
length_bp: 1018
variable_sites: 6
pi: 0.00378
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-ycf15_p1	TGGCAACTTTACGTAAGGCA	GAAAGGACTCACTAAGCCGG	45.0	55.0	57.7	58.0	1057-1094 bp	1.0	81.0
rps12-ycf15_p2	TGGCAACTTTACGTAAGGCA	CACGGTACAATCCTCTTCCC	45.0	55.0	57.7	57.7	1146-1183 bp	1.0	78.3
rps12-ycf15_p3	ACGTAAGGCAGAGTTTGGTT	GAAAGGACTCACTAAGCCGG	45.0	55.0	57.4	58.0	1047-1084 bp	1.0	78.0
rps12-ycf15_p4	TGGCAACTTTACGTAAGGCA	TCACTAAGCCGGGATCACTA	45.0	50.0	57.7	57.6	1049-1086 bp	1.0	77.5
rps12-ycf15_p5	TGGCAACTTTACGTAAGGCA	CCCTTTCTCCTCTGTCCACA	45.0	55.0	57.7	58.6	1118-1155 bp	1.0	75.7