Hotspot

rpl33-rps18

LSC · 67633-67816 · 184 bp

Back to Cicerbita

Pi 0.012

MarkerSeek score 63.70

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 184
variable_sites: 3
pi: 0.012
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGATTCACGGGGAGGTAAAG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.1	57.7	289-296 bp	1.0	80.2
rpl33-rps18_p2	GGCACAATACGCCTAATCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.1	57.7	349-356 bp	1.0	79.1
rpl33-rps18_p3	ACTCAAAAGAACCGGCACAA	TCAATTCGATCCCCCGATTG	45.0	50.0	58.2	57.7	362-369 bp	1.0	78.2
rpl33-rps18_p4	CGATTCACGGGGAGGTAAAG	ATTCGATCCCCCGATTGGAT	55.0	50.0	58.1	58.6	286-293 bp	1.0	77.2
rpl33-rps18_p5	GGCACAATACGCCTAATCGA	ATTCGATCCCCCGATTGGAT	50.0	50.0	58.1	58.6	346-353 bp	1.0	76.1