Hotspot

ycf4-cemA

LSC · 60255-61039 · 785 bp

Back to Cicerbita

Pi 0.0115

MarkerSeek score 67.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 785
variable_sites: 15
pi: 0.0115
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	964-973 bp	1.0	81.0
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	896-905 bp	1.0	80.6
ycf4-cemA_p3	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	928-937 bp	1.0	80.5
ycf4-cemA_p4	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	910-919 bp	1.0	79.9
ycf4-cemA_p5	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	908-917 bp	1.0	79.6