Hotspot

trnG-UCC-trnT

LSC · 30936-31113 · 178 bp

Back to Cicerbita

Pi 0.0365

MarkerSeek score 74.60

Primer available yes

Label trnG-UCC-trnT

Feature Metadata

label_name: trnG-UCC-trnT
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 10
pi: 0.0365
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT_p1	AATCAAACCGAAAGACCCCT	ATTTGAACCGAGGGCTTACG	45.0	50.0	57.0	58.3	304-328 bp	1.0	76.4
trnG-UCC-trnT_p2	ATCAAACCGAAAGACCCCTT	ATTTGAACCGAGGGCTTACG	45.0	50.0	57.0	58.3	303-327 bp	1.0	76.4
trnG-UCC-trnT_p3	AATCAAACCGAAAGACCCCT	GGATTTGAACCGAGGGCTTA	45.0	50.0	57.0	57.6	306-330 bp	1.0	75.2
trnG-UCC-trnT_p4	ATCAAACCGAAAGACCCCTT	GGATTTGAACCGAGGGCTTA	45.0	50.0	57.0	57.6	305-329 bp	1.0	75.2
trnG-UCC-trnT_p5	GAATCAAACCGAAAGACCCCT	ATTTGAACCGAGGGCTTACG	47.6	50.0	58.2	58.3	305-329 bp	1.0	74.5