Hotspot

ycf1

Genome · 20129-25363 · 5235 bp

Pi 0.0322

MarkerSeek score 63.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5235
variable_sites: 277
pi: 0.0322
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTCGGGAGAAATCTGGTTCG	AACAAGTTCCTCGATGGTCA	55.0	45.0	58.1	56.8	216-282 bp	1.0	81.7
ycf1_p2	CTCGGGAGAAATCTGGTTCG	ACGTTTTCGAGGACAAATCCA	55.0	42.9	58.1	58.2	602-668 bp	1.0	81.1
ycf1_p3	TGTCTTCAGCCTCGTCTTTG	ACGTTTTCGAGGACAAATCCA	50.0	42.9	57.8	58.2	860-926 bp	1.0	80.1
ycf1_p4	TGTGCCAAGGTTTCAGAACA	ACGTTTTCGAGGACAAATCCA	45.0	42.9	57.9	58.2	1175-1241 bp	1.0	79.2
ycf1_p5	TGCTTTCCCAGGATTCTTCG	ACGTTTTCGAGGACAAATCCA	50.0	42.9	57.9	58.2	953 bp	0.25	39.8