Hotspot

atpH-atpI

Genome · 83823-85080 · 1258 bp

Back to Cicer

Pi 0.0302
MarkerSeek score 76.00
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
Genome
length_bp
1258
variable_sites
61
pi
0.0302
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 GCAGCAATAACAGAAGCAGC GTGAATCCATGGAAGGGCAT 50.0 50.0 58.1 57.9 1222-1322 bp 1.0 86.4
atpH-atpI_p2 ATAGAAGCAAGTCCAACGGC GTGAATCCATGGAAGGGCAT 50.0 50.0 58.3 57.9 1249-1349 bp 1.0 85.0
atpH-atpI_p3 GCAGCAATAACAGAAGCAGC GAATCCATGGAAGGGCATCA 50.0 50.0 58.1 57.6 1220-1320 bp 1.0 84.1
atpH-atpI_p4 CTGTGCCTTGACCAATTCCA GTGAATCCATGGAAGGGCAT 50.0 50.0 58.4 57.9 1275-1375 bp 1.0 84.0
atpH-atpI_p5 TGTGCCTTGACCAATTCCAG GTGAATCCATGGAAGGGCAT 50.0 50.0 58.4 57.9 1274-1374 bp 1.0 84.0