Hotspot

trnR-UCU-atpA

Genome · 80023-80213 · 191 bp

Back to Cicer

Pi 0.0515
MarkerSeek score 82.90
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
Genome
length_bp
191
variable_sites
16
pi
0.0515
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 TTCAAATCCTATTGGGCGCA TTCACTGGGGAAGCAGAAAC 45.0 50.0 57.9 58.0 301-310 bp 1.0 85.6
trnR-UCU-atpA_p2 TTCAAATCCTATTGGGCGCA CATTCACTGGGGAAGCAGAA 45.0 50.0 57.9 57.8 303-312 bp 1.0 84.0
trnR-UCU-atpA_p3 TTCAAATCCTATTGGGCGCA AAGACATTCACTGGGGAAGC 45.0 50.0 57.9 57.8 307-316 bp 1.0 83.9
trnR-UCU-atpA_p4 TTCAAATCCTATTGGGCGCA GGGGAAGCAGAAACCCTTTT 45.0 50.0 57.9 58.3 295-304 bp 1.0 83.6
trnR-UCU-atpA_p5 AAGAAAAGCGCCCATTGTCT TTCACTGGGGAAGCAGAAAC 45.0 50.0 58.4 58.0 360-369 bp 1.0 82.5