Hotspot

trnQ-UUG-psbK

Genome · 76638-77274 · 637 bp

Back to Cicer

Pi 0.0409
MarkerSeek score 76.80
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
Genome
length_bp
637
variable_sites
44
pi
0.0409
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 GACCAAAACCCGTTGCCTTA ACTTGAATAAAGGGCAGAGTCA 50.0 40.9 58.7 57.4 732-761 bp 1.0 58.7
trnQ-UUG-psbK_p2 CGAATAACGGGACCAAAACC ACTTGAATAAAGGGCAGAGTCA 50.0 40.9 57.1 57.4 742-771 bp 1.0 56.8
trnQ-UUG-psbK_p3 ACCAAAACCCGTTGCCTTAC ACTTGAATAAAGGGCAGAGTCA 50.0 40.9 59.0 57.4 731-760 bp 1.0 56.4
trnQ-UUG-psbK_p4 GACCAAAACCCGTTGCCTTA ACTTGAATAAAGGGCAGAGTCAA 50.0 39.1 58.7 58.0 732-761 bp 1.0 55.7
trnQ-UUG-psbK_p5 CGAATAACGGGACCAAAACC ACTTGAATAAAGGGCAGAGTCAA 50.0 39.1 57.1 58.0 742-771 bp 1.0 53.8