Hotspot

rpl20-rps18

Genome · 64301-64570 · 270 bp

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Pi 0.0431
MarkerSeek score 75.50
Primer available yes
Label rpl20-rps18

Feature Metadata

label_name
rpl20-rps18
feature_type
igs
region
Genome
length_bp
270
variable_sites
20
pi
0.0431
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps18_p1 TCTGTACAAGAAGCAATTGC AGCGAGTCGATCACTAGAAC 40.0 50.0 54.2 56.9 422-430 bp 1.0 45.9
rpl20-rps18_p2 TCTGTACAAGAAGCAATTGC GCGAGTCGATCACTAGAACT 40.0 50.0 54.2 56.9 421-429 bp 1.0 45.9
rpl20-rps18_p3 TCTGTACAAGAAGCAATTGC AGCGAGTCGATCACTAGAACT 40.0 47.6 54.2 58.4 422-430 bp 1.0 45.1
rpl20-rps18_p4 TCTGTACAAGAAGCAATTGC AAGCGAGTCGATCACTAGAAC 40.0 47.6 54.2 57.6 423-431 bp 1.0 45.1
rpl20-rps18_p5 ATCTGTACAAGAAGCAATTGC GCGAGTCGATCACTAGAACT 38.1 50.0 54.8 56.9 422-430 bp 1.0 45.1