Hotspot

trnN-GUU-trnR-ACG

Genome · 26005-26614 · 610 bp

Back to Cicer

Pi 0.0533
MarkerSeek score 86.30
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
Genome
length_bp
610
variable_sites
53
pi
0.0533
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 750-768 bp 1.0 85.4
trnN-GUU-trnR-ACG_p2 ATTTGCCGTTGTTCCTCAGT CAGAGGATTAGAGCACGTGG 45.0 55.0 58.0 57.8 755-773 bp 1.0 85.2
trnN-GUU-trnR-ACG_p3 TTGCCGTTGTTCCTCAGTAG CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 753-771 bp 1.0 84.0
trnN-GUU-trnR-ACG_p4 TTCCTCAGTAGCTCAGTGGT CAGAGGATTAGAGCACGTGG 50.0 55.0 57.7 57.8 744-762 bp 1.0 82.9
trnN-GUU-trnR-ACG_p5 CCGATTGGTCGTAGGTTCAA CAGAGGATTAGAGCACGTGG 50.0 55.0 57.6 57.8 706-724 bp 1.0 82.8