Hotspot

rbcL-trnK-UUU

Genome · 1402-2065 · 664 bp

Back to Cicer

Pi 0.0307
MarkerSeek score 77.70
Primer available yes
Label rbcL-trnK-UUU

Feature Metadata

label_name
rbcL-trnK-UUU
feature_type
igs
region
Genome
length_bp
664
variable_sites
34
pi
0.0307
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-trnK-UUU_p1 AGTCCTGAATTAGCTGCTGC TGGAGTCGCACTTAAAAGCC 50.0 50.0 58.0 58.5 783-818 bp 1.0 80.0
rbcL-trnK-UUU_p2 AGTCCTGAATTAGCTGCTGC AACCCTACCGATGGTATGGA 50.0 50.0 58.0 57.5 836-871 bp 1.0 79.7
rbcL-trnK-UUU_p3 AGTCCTGAATTAGCTGCTGC AGTCGCACTTAAAAGCCGAG 50.0 50.0 58.0 58.6 780-815 bp 1.0 79.3
rbcL-trnK-UUU_p4 AGTCCTGAATTAGCTGCTGC AGTCGTGGTCTTACAAACCC 50.0 50.0 58.0 57.5 851-886 bp 1.0 79.2
rbcL-trnK-UUU_p5 AGTCCTGAATTAGCTGCTGC GTCGTGGTCTTACAAACCCT 50.0 50.0 58.0 57.5 850-885 bp 1.0 79.2