Hotspot

rps12-rrn16

Genome · 148114-155493 · 7380 bp

Back to Cibotium

Pi 0.0035

MarkerSeek score 58.80

Primer available yes

Label rps12-rrn16

Feature Metadata

label_name: rps12-rrn16
feature_type: igs
region: Genome
length_bp: 7380
variable_sites: 24
pi: 0.0035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rrn16_p1	GGGACAGTGTCGATAGTGTC	ACCCGTTTGCTTGATGTCTC	55.0	50.0	57.4	58.5	188-567 bp	1.0	76.7
rps12-rrn16_p2	GGGACAGTGTCGATAGTGTC	CCCGTTTGCTTGATGTCTCA	55.0	50.0	57.4	58.5	187-566 bp	1.0	76.7
rps12-rrn16_p3	GGGACAGTGTCGATAGTGTC	TTAGACAGAACAGCCAACGC	55.0	50.0	57.4	58.5	166-545 bp	1.0	76.7
rps12-rrn16_p4	GGGACAGTGTCGATAGTGTC	CCGTTTGCTTGATGTCTCAC	55.0	50.0	57.4	57.4	186-565 bp	1.0	75.7
rps12-rrn16_p5	GGGGACAGTGTCGATAGTGT	ACCCGTTTGCTTGATGTCTC	55.0	50.0	58.8	58.5	189-568 bp	1.0	74.7