Hotspot

ndhB-trnR-ACG

Genome · 86718-88554 · 1837 bp

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Pi 0.00332
MarkerSeek score 61.40
Primer available yes
Label ndhB-trnR-ACG

Feature Metadata

label_name
ndhB-trnR-ACG
feature_type
igs
region
Genome
length_bp
1837
variable_sites
6
pi
0.00332
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnR-ACG_p1 TCGGGATCACACAGAATACCT AAAAGTGGTTCCTGGAACGG 47.6 50.0 58.3 58.3 1974-2043 bp 1.0 68.8
ndhB-trnR-ACG_p2 TCGGGATCACACAGAATACC AAAAGTGGTTCCTGGAACGG 50.0 50.0 56.7 58.3 1974-2043 bp 1.0 68.7
ndhB-trnR-ACG_p3 CGGGATCACACAGAATACCT AAAAGTGGTTCCTGGAACGG 50.0 50.0 56.7 58.3 1973-2042 bp 1.0 68.6
ndhB-trnR-ACG_p4 CGGGATCACACAGAATACCTT AAAAGTGGTTCCTGGAACGG 47.6 50.0 57.5 58.3 1973-2042 bp 1.0 66.5
ndhB-trnR-ACG_p5 CCATTATCGGGATCACACAGA AAAAGTGGTTCCTGGAACGG 47.6 50.0 57.2 58.3 1980-2049 bp 1.0 64.8