Hotspot

rps4-trnL-CAA

Genome · 47171-47848 · 678 bp

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Pi 0.00737

MarkerSeek score 63.00

Primer available yes

Label rps4-trnL-CAA

Feature Metadata

label_name: rps4-trnL-CAA
feature_type: igs
region: Genome
length_bp: 678
variable_sites: 5
pi: 0.00737
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-CAA_p1	CCTGTAAGCCCGGGTAAATC	CTGAATCTGGGAGCTGTCAC	55.0	55.0	58.0	58.0	834-836 bp	1.0	80.0
rps4-trnL-CAA_p2	CTGTAAGCCCGGGTAAATCC	CTGAATCTGGGAGCTGTCAC	55.0	55.0	58.0	58.0	833-835 bp	1.0	80.0
rps4-trnL-CAA_p3	ATGCTTTACCTGTAAGCCCG	CTGAATCTGGGAGCTGTCAC	50.0	55.0	58.0	58.0	842-844 bp	1.0	80.0
rps4-trnL-CAA_p4	TACCTGTAAGCCCGGGTAAA	CTGAATCTGGGAGCTGTCAC	50.0	55.0	58.1	58.0	836-838 bp	1.0	79.9
rps4-trnL-CAA_p5	TTTACCTGTAAGCCCGGGTA	CTGAATCTGGGAGCTGTCAC	50.0	55.0	58.1	58.0	838-840 bp	1.0	79.9