Hotspot

psbD-trnT-GGU

Genome · 35893-37462 · 1570 bp

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Pi 0.0051

MarkerSeek score 61.50

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1570
variable_sites: 8
pi: 0.0051
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	AGACCAGACCAACCTACGAA	GGCCCTTTTAACTCAGTGGT	50.0	50.0	58.0	57.7	1750 bp	1.0	78.0
psbD-trnT-GGU_p2	TTTTCCAATGGCGATGGTCA	GGCCCTTTTAACTCAGTGGT	45.0	50.0	58.1	57.7	1664 bp	1.0	77.4
psbD-trnT-GGU_p3	TTTCCAATGGCGATGGTCAT	GGCCCTTTTAACTCAGTGGT	45.0	50.0	57.9	57.7	1663 bp	1.0	76.9
psbD-trnT-GGU_p4	AGACCAGACCAACCTACGAA	AGGCCCTTTTAACTCAGTGG	50.0	50.0	58.0	57.4	1751 bp	1.0	75.6
psbD-trnT-GGU_p5	TTTTCCAATGGCGATGGTCA	AGGCCCTTTTAACTCAGTGG	45.0	50.0	58.1	57.4	1665 bp	1.0	75.0